5MGX
The structure of FKBP38 in complex with the MEEVD tetratricopeptide binding-motif of Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.290, 105.640, 100.190 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.180 |
| R-factor | 0.249 |
| Rwork | 0.246 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AWG and 2FBN |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.111 | 0.492 |
| Number of reflections | 76276 | |
| <I/σ(I)> | 5 | 1.5 |
| Completeness [%] | 95.9 | 96 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.947 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 0.02 M sodium potassium phosphate, 20 % w/v PEG 3350 |
