5MGH
Crystal structure of pathogenic mutants of human mitochodnrial PheRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.045, 89.355, 97.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.901 - 1.870 |
R-factor | 0.1992 |
Rwork | 0.198 |
R-free | 0.22990 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.899 |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.901 |
High resolution limit [Å] | 1.870 |
Number of reflections | 40255 |
<I/σ(I)> | 25.2 |
Completeness [%] | 99.6 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 2 mM DTT, 100 mM bis-Tris propane pH 7 and 1.8 M sodium acetate pH 7 |