5MFM
Designed armadillo repeat protein peptide fusion YIIIM6AII_GS11_(KR)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.760, 89.390, 123.850 |
| Unit cell angles | 90.00, 97.07, 90.00 |
Refinement procedure
| Resolution | 44.690 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 1.630 |
| Number of reflections | 77719 | |
| <I/σ(I)> | 8.67 | 1.07 |
| Completeness [%] | 98.6 | 99.1 |
| Redundancy | 4.2 | 4.3 |
| CC(1/2) | 0.998 | 0.156 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 37.5% (MPD/PEG1000/PEG3350), 0.03 M MgCl2, 0.03 M CaCl2, Bicine/TRIS pH 8.5 |
