5MFH
Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.480, 103.790, 134.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.390 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mfe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.543 | 9.230 |
| Number of reflections | 77426 | |
| <I/σ(I)> | 5.52 | 0.29 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.1 | 11 |
| CC(1/2) | 0.990 | 0.108 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 5% PEG8000, 27.27% MPD, 0.1 M Na Cacodylate pH6.7 |
