5MEQ
Human Leukocyte Antigen A02 presenting ILAKFLHTL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.340, 81.440, 56.770 |
| Unit cell angles | 90.00, 113.53, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.270 |
| R-factor | 0.2065 |
| Rwork | 0.203 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i4w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.976 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | |
| High resolution limit [Å] | 2.270 | |
| Rmerge | 0.101 | 0.751 |
| Rmeas | 0.119 | 0.876 |
| Number of reflections | 20523 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium sulphate, 0.1M HEPES pH7, 20% PEG 8000 |
