5MEA

Crystal structure of yeast Cdt1 (N terminal and middle domain), form 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	47.510, 89.350, 121.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.948 - 2.152
R-factor	0.2056
Rwork	0.203
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	the structure i'm depositing with this one - that was solved by SAD (SeMet) - sorry i don't have a pdb code yet...
RMSD bond length	0.002
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.260
High resolution limit [Å]	2.150	2.150
Rmerge	0.098	1.060
Number of reflections	28350
<I/σ(I)>	13.1	2
Completeness [%]	98.7	95.4
Redundancy	5.8	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	10.5% PEG 8K, 18% glycerol, 0.5M lithium sulphate