5ME6
Crystal Structure of eiF4E from C. melo bound to a CAP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.699, 108.868, 122.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.26330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.976 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.475 | 47.087 | 2.700 |
| High resolution limit [Å] | 2.564 | 8.110 | 2.560 |
| Rmerge | 0.142 | 0.881 | |
| Rmeas | 0.274 | 0.160 | 0.984 |
| Rpim | 0.120 | 0.072 | 0.428 |
| Total number of observations | 125316 | 4107 | 13561 |
| Number of reflections | 24722 | ||
| <I/σ(I)> | 4.2 | 7.4 | 1.5 |
| Completeness [%] | 96.7 | 99 | 78.2 |
| Redundancy | 5.1 | 4.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | sodium potassium tartrate |
