5ME6

Crystal Structure of eiF4E from C. melo bound to a CAP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	57.699, 108.868, 122.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.2222
Rwork	0.220
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.976
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.475	47.087	2.700
High resolution limit [Å]	2.564	8.110	2.560
Rmerge		0.142	0.881
Rmeas	0.274	0.160	0.984
Rpim	0.120	0.072	0.428
Total number of observations	125316	4107	13561
Number of reflections	24722
<I/σ(I)>	4.2	7.4	1.5
Completeness [%]	96.7	99	78.2
Redundancy	5.1	4.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	sodium potassium tartrate