5MDI
Crystal structure of TDP-43 N-terminal domain at 2.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 63 |
| Unit cell lengths | 89.518, 89.518, 49.153 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.760 - 2.100 |
| R-factor | 0.16646 |
| Rwork | 0.164 |
| R-free | 0.20583 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.158 | 4.950 |
| Number of reflections | 32038 | |
| <I/σ(I)> | 13.13 | 0.57 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.9 |
| CC(1/2) | 0.206 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M sodium cacodylate pH 6.5, 1.4 M sodium acetate |
