5MC9
Crystal structure of the heterotrimeric integrin-binding region of laminin-111
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.760, 98.360, 135.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.900 - 2.130 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjs |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.900 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.092 | 0.780 |
| Number of reflections | 47309 | |
| <I/σ(I)> | 11.5 | 1.9 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.997 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.02 M magnesium chloride hexahydrate, 0.1 M Na-HEPES (pH 7.5), 22% w/v poly(acrylic acid sodium salt) 5100 |
