5MAD
GFP-binding DARPin 3G61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.420, 83.070, 162.000 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 48.760 - 1.530 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 1.670 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.047 | 0.169 |
| Number of reflections | 237243 | |
| <I/σ(I)> | 15.79 | 1.39 |
| Completeness [%] | 98.8 | |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.999 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Sodium citrate tribasic, pH 5.5, 30% PEG 4000, 0.2 M Ammonium acetate |
