5MA9
GFP-binding DARPin fusion gc_R11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.880, 89.890, 90.040 |
| Unit cell angles | 95.84, 116.53, 92.35 |
Refinement procedure
| Resolution | 44.682 - 1.570 |
| R-factor | 0.1606 |
| Rwork | 0.158 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.682 | 1.610 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.046 | 0.569 |
| Number of reflections | 302440 | |
| <I/σ(I)> | 14.03 | 2.6 |
| Completeness [%] | 95.2 | 94.4 |
| Redundancy | 3.4 | 3.62 |
| CC(1/2) | 0.990 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M TRIS pH 8.5, 30% w/v PEG 4000, 0.2 M LiSO4 |
