5MA3
GFP-binding DARPin fusion gc_R11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.850, 90.610, 60.920 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 48.979 - 1.700 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.979 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.105 | 3.440 |
| Number of reflections | 67771 | |
| <I/σ(I)> | 10.47 | 0.98 |
| Completeness [%] | 97.6 | 98.6 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.997 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate pH 6.5, 30% w/v PEG 8000 |
