5M9X

Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	83.103, 83.103, 157.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.065 - 2.349
R-factor	0.2598
Rwork	0.258
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.623
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2443: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	2.349
Number of reflections	23334
<I/σ(I)>	20.88
Completeness [%]	98.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	289	PEG 1000, 150MM NACL, MES, 2%