5M9T
Human angiogenin ALS variant H114R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.426, 81.426, 85.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.500 - 2.200 |
| R-factor | 0.18556 |
| Rwork | 0.182 |
| R-free | 0.23596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.598 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.500 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16435 | |
| <I/σ(I)> | 12.1 | 1.8 |
| Completeness [%] | 95.8 | 75.9 |
| Redundancy | 9.5 | 3.6 |
| CC(1/2) | 0.994 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.01 M Na borate 1.1 M Na citrate |
