5M9Q
Human angiogenin PD variant R95Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.573, 37.393, 43.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 1.350 |
| R-factor | 0.15209 |
| Rwork | 0.151 |
| R-free | 0.17930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.750 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.600 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 29013 | |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 96.6 | 71.6 |
| Redundancy | 12 | 7 |
| CC(1/2) | 0.998 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M ammonium sulphate 0.1 M Na cacodylate 30% PEG 8000 |
