5M9P
Human angiogenin ALS variant T80S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.425, 37.335, 42.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.760 - 1.800 |
| R-factor | 0.24195 |
| Rwork | 0.238 |
| R-free | 0.28522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.458 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 12236 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 95.7 | 77.9 |
| Redundancy | 6.4 | 3.1 |
| CC(1/2) | 0.984 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.2 M ammonium sulphate 0.1 M bis-tris propane 25% PEG 3350 |
