5M9O
Crystal structure of human SND1 extended Tudor domain in complex with a symmetrically dimethylated E2F peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.243, 80.626, 39.289 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 28.140 - 1.450 |
| R-factor | 0.23039 |
| Rwork | 0.229 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3omc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.140 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.037 | 0.292 |
| Number of reflections | 38367 | |
| <I/σ(I)> | 16.2 | 2.8 |
| Completeness [%] | 96.0 | 69.9 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 10 % PEG 1000, 10% PEG 8000 |
