5M9N
Crystal structure of human TDRD1 extended Tudor domain in complex with a symmetrically dimethylated E2F peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0282 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.910, 52.962, 59.619 |
| Unit cell angles | 64.94, 88.85, 67.67 |
Refinement procedure
| Resolution | 29.340 - 1.950 |
| R-factor | 0.21482 |
| Rwork | 0.212 |
| R-free | 0.26035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9x |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.034 | 0.411 |
| Number of reflections | 30788 | |
| <I/σ(I)> | 16.7 | 2.3 |
| Completeness [%] | 96.9 | 89.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 30% PEG4000, 0.2M MgCl2, 0.1 M Tris pH 8.5 |
