5M9M
Human angiogenin PD variant Q77P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.170, 50.337, 62.190 |
| Unit cell angles | 103.42, 103.59, 110.40 |
Refinement procedure
| Resolution | 56.750 - 1.650 |
| R-factor | 0.23296 |
| Rwork | 0.232 |
| R-free | 0.27046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 60548 | |
| <I/σ(I)> | 7.3 | 1.9 |
| Completeness [%] | 96.6 | 70.4 |
| Redundancy | 14.8 | 10.8 |
| CC(1/2) | 0.988 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M ammonium sulphate 0.1 M bis-tris methane 25% PEG 3350 |
