5M9E

Interactions between the Mal3 EB1-like domain and Dis1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-05-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 65 2 2
Unit cell lengths	93.305, 93.305, 196.545
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.800 - 2.830
R-factor	0.2691
Rwork	0.266
R-free	0.32450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m97
RMSD bond length	0.012
RMSD bond angle	1.524
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.800	2.931
High resolution limit [Å]	2.830	2.830
Rmerge	0.177	2.937
Number of reflections	12733
<I/σ(I)>	9.13	1.3
Completeness [%]	97.0	100
Redundancy	18.3	19
CC(1/2)		0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M MgCl2, 0.1 M HEPES pH 7, 20% PEG 6K