5M99
Functional Characterization and Crystal Structure of Thermostable Amylase from Thermotoga petrophila, reveals High Thermostability and an Archaic form of Dimerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 |
| Unit cell lengths | 153.237, 153.237, 143.056 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.159 - 1.960 |
| R-factor | 0.1525 |
| Rwork | 0.152 |
| R-free | 0.18400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.160 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 136221 | |
| <I/σ(I)> | 16.8 | 2.4 |
| Completeness [%] | 95.6 | 99 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1 M Tris, 1.2 M Na/K tartrate |
