5M98
Crystal structure of urate oxidase from zebrafish
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.341, 127.415, 132.604 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 83.200 - 2.800 |
| R-factor | 0.20625 |
| Rwork | 0.204 |
| R-free | 0.24168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.695 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.300 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.168 | 0.427 |
| Number of reflections | 64855 | |
| <I/σ(I)> | 5.2 | 2.2 |
| Completeness [%] | 94.9 | 89 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | Tris-HCl 0.1M, pH 8.5, 8% PEG 8000 |
