5M8M

Crystal structure of human tyrosinase related protein 1 in complex with kojic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	90.255, 141.535, 192.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.650
R-factor	0.1993
Rwork	0.197
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	2.650
Number of reflections	71959
<I/σ(I)>	9
Completeness [%]	99.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000