5M8L
Crystal structure of human tyrosinase related protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.424, 141.758, 191.741 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.466 - 2.350 |
R-factor | 0.2161 |
Rwork | 0.214 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nm8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.107 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.980 |
High resolution limit [Å] | 2.350 |
Number of reflections | 102816 |
<I/σ(I)> | 6.6 |
Completeness [%] | 99.7 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000 |