5M8D
Tubulin MTD265-R1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.180, 157.020, 179.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.101 - 2.250 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20990 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.101 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.117 | 1.844 |
| Number of reflections | 139439 | |
| <I/σ(I)> | 17 | 1.4 |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 13.4 | 11.6 |
| CC(1/2) | 0.999 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 3% PEG 4000, 4-6% GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 30 MM CALCIUM CHLORIDE, 100 MM MES/IMIDAZOLE |
