5M8C
Spliceosome component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.220, 100.140, 65.340 |
| Unit cell angles | 90.00, 92.33, 90.00 |
Refinement procedure
| Resolution | 48.992 - 2.300 |
| R-factor | 0.1805 |
| Rwork | 0.177 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lrv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.992 | 48.992 | 2.400 |
| High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
| Rmerge | 0.069 | 0.023 | 0.376 |
| Rmeas | 0.082 | 0.027 | 0.448 |
| Total number of observations | 107782 | ||
| Number of reflections | 31956 | 407 | 3784 |
| <I/σ(I)> | 12.35 | 40.1 | 3.04 |
| Completeness [%] | 99.3 | 97.8 | 99 |
| Redundancy | 3.373 | 3.462 | 3.357 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M MOPS pH 7.0 12% PEG 4000 |
