5M84
Translation initiation factor 4E in complex with (SP)-m2(7,2'O)GppSpA mRNA 5' cap analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 46.946, 59.763, 149.428 |
Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
Resolution | 37.163 - 1.850 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.048 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.063 | 0.823 |
Number of reflections | 35357 | |
<I/σ(I)> | 14.16 | 1.64 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 4.664 | 4.722 |
CC(1/2) | 0.999 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 16% PEG 10000, 0.2M CH3COONa, 0.1M TRIS pH 8.0 |