5M81

Translation initiation factor 4E in complex with (SP)-iPr-m7GppSpG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	38.100, 38.120, 147.240
Unit cell angles	88.77, 95.82, 103.44

Refinement procedure

Resolution	48.827 - 1.900
R-factor	0.1785
Rwork	0.177
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.078	0.587
Number of reflections	62076
<I/σ(I)>	11.88	1.99
Completeness [%]	98.2	97.6
Redundancy	2.919	2.971
CC(1/2)	0.997	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	12% PEG 10000, 0.2M CH3COONa, 0.1M TRIS pH 8.0