5M7Z
Translation initiation factor 4E in complex with (SP)-m2(7,2'O)GppSepG mRNA 5' cap analog (beta-Se-ARCA D2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979570 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.140, 38.140, 147.180 |
| Unit cell angles | 88.28, 84.07, 76.64 |
Refinement procedure
| Resolution | 37.107 - 1.689 |
| R-factor | 0.2195 |
| Rwork | 0.219 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.075 | 0.606 |
| Number of reflections | 88368 | |
| <I/σ(I)> | 10.84 | 2.01 |
| Completeness [%] | 98.1 | 94.8 |
| Redundancy | 3.756 | 3.704 |
| CC(1/2) | 0.996 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 18% PEG 5000 MME, 0.2M HCOONa, 0.1M BICINE pH 8.5 |
