5M7Y
Crystal structure of GH125 1,6-alpha-mannosidase mutant from Clostridium perfringens in complex with 1,6-alpha-mannotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.414, 44.016, 85.079 |
| Unit cell angles | 90.00, 96.59, 90.00 |
Refinement procedure
| Resolution | 51.820 - 1.550 |
| R-factor | 0.1487 |
| Rwork | 0.147 |
| R-free | 0.18010 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.067 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.820 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.035 | 0.400 |
| Number of reflections | 263043 | |
| <I/σ(I)> | 21.8 | 3.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 3.6 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 200 mM MgCl2, 100 mM HEPES pH7.0, 27% polyethylene glycol 3350 |
