5M6Z
The X-ray structure of human M189I PGK-1 mutant in partially closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.872899 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.880, 106.121, 50.472 |
| Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
| Resolution | 53.060 - 1.670 |
| R-factor | 0.18488 |
| Rwork | 0.183 |
| R-free | 0.22295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.558 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.054 | 0.675 |
| Number of reflections | 42601 | |
| <I/σ(I)> | 15.08 | 1.89 |
| Completeness [%] | 97.5 | 94.1 |
| Redundancy | 4.26 | 4.18 |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 294 | 2.5 M Na//KPO4, pH=8.6 |
