5M6J

Crystal structure of nitrophorin 7 E27V mutant from Rhodnius prolixus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-26
Detector	RAYONIX MX225HE
Wavelength(s)	0.91841
Spacegroup name	P 1 21 1
Unit cell lengths	38.228, 66.885, 38.714
Unit cell angles	90.00, 116.57, 90.00

Refinement procedure

Resolution	34.626 - 1.700
R-factor	0.1635
Rwork	0.161
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xmc
RMSD bond length	0.006
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (2011_08_24_1020)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.630	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.057	0.488
Number of reflections	72215
<I/σ(I)>	17.6	3
Completeness [%]	96.6	95.3
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	NaOAc/HOAc