5M6I
Crystal structure of non-cardiotoxic Bence-Jones light chain dimer M8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96862 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 229.378, 229.378, 64.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.560 - 2.200 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m6a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.340 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.271 | 2.548 |
| Number of reflections | 43679 | |
| <I/σ(I)> | 12.4 | 2.3 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 14.2 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2 M Sodium acetate, 2.0 M NaCl |
