5M62

Structure of the Mus musclus Langerin carbohydrate recognition domain in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 3
Unit cell lengths	144.420, 144.420, 144.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.670 - 1.700
R-factor	0.14485
Rwork	0.144
R-free	0.16436
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k8y
RMSD bond length	0.009
RMSD bond angle	1.398
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.700	1.700
Rmeas	0.116	1.783
Number of reflections	54929
<I/σ(I)>	28.2	3.2
Completeness [%]	100.0	100
Redundancy	40.4	41.1
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MES, pH 6.0, 30% (v/v) polyethylene glycol 600, 5% (w/v) polyethylene glycol 1000, and 10% (v/v) glycerol, 5% Glucose