5M60

Chaetomium thermophilum beta-1-3-glucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	63.661, 83.535, 65.171
Unit cell angles	90.00, 93.10, 90.00

Refinement procedure

Resolution	44.291 - 1.500
R-factor	0.1582
Rwork	0.157
R-free	0.18160
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.865
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.300	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.009	0.765
Number of reflections	106298
<I/σ(I)>	10.3	1.8
Completeness [%]	99.8	96.4
Redundancy	4.9	5.1
CC(1/2)	0.998	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	289	1.8 M Na/K phosphate