5M5V
Clathrin heavy chain N-terminal domain bound to a clathrin-box motif from hepatitis D virus large antigen (clade 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.900, 131.230, 78.450 |
| Unit cell angles | 90.00, 115.86, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.960 |
| R-factor | 0.17037 |
| Rwork | 0.169 |
| R-free | 0.19343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.917 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.081 | 0.581 |
| Number of reflections | 79976 | |
| <I/σ(I)> | 7.2 | 1.5 |
| Completeness [%] | 94.6 | 95.9 |
| Redundancy | 2.5 | 2.3 |
| CC(1/2) | 0.996 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 200 nL protein:peptide (20 mg/mL NTD and 3.6 mM peptide) plus 400 nL reservoir equilibrated against a 80 uL reservoir of 1.75 M sodium malonate pH 7.0 |
