5M5R
Clathrin heavy chain N-terminal domain bound to beta2 adaptin clathrin box motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92818 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.144, 133.177, 77.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.100 - 1.760 |
| R-factor | 0.17725 |
| Rwork | 0.176 |
| R-free | 0.20516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.719 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.053 | 1.538 |
| Number of reflections | 52951 | |
| <I/σ(I)> | 15.8 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7 |
| CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 1 uL protein plus peptide mix (14 mg/mL NTD and 3.4 mM peptide) plus 2 uL reservoir equilibrated against a 200 uL reservoir of 0.94 M sodium malonate pH 6.7 |
