5M4Q
Crystal Structure of Wild-Type Human Prolidase with Mn ions and Pro ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.429, 107.005, 216.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.650 - 1.730 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.18000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.074 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.948 | 1.830 |
| High resolution limit [Å] | 1.728 | 1.730 |
| Rmerge | 0.082 | 1.333 |
| Number of reflections | 124682 | |
| <I/σ(I)> | 17.11 | 1.39 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 6.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 10mM NaBorate, 690-760mM NaCitrate |
