5M48

Coiled coil domain of Rtt103p

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979, 0.9792, 0.9919, 0.9713
Spacegroup name	F 41 3 2
Unit cell lengths	217.141, 217.141, 217.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.554 - 2.593
R-factor	0.2474
Rwork	0.245
R-free	0.26400
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9-1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.540	2.681
High resolution limit [Å]	2.593	2.589
Number of reflections	14200
<I/σ(I)>	21.9
Completeness [%]	100.0	99.9
Redundancy	29.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Purified protein was dialysed to 25mM Tris 200mM NaCl 1mM BME, pH = 8.0 (4oC) and concentrated to 6mg/ml. Protein was crystallized in 3.75M sodium formate.