5M3X
Crystal structure of human angiotensin I-deleted angiotensinogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 86.300, 41.040, 129.950 |
| Unit cell angles | 90.00, 107.82, 90.00 |
Refinement procedure
| Resolution | 43.440 - 2.630 |
| R-factor | 0.2439 |
| Rwork | 0.242 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wxw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.559 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.139 | 0.801 |
| Number of reflections | 26239 | |
| <I/σ(I)> | 6.9 | 1.3 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.7M Ammonium Sulfate, 0.1M Citric Acid, pH3.6 |
