5M3J

Influenza B polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 32 2 1
Unit cell lengths	200.160, 200.160, 250.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.070 - 3.500
R-factor	0.2401
Rwork	0.239
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsa
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.191	2.220
Number of reflections	73405
<I/σ(I)>	8.38	1.02
Completeness [%]	99.9	99.9
Redundancy	8.54	8.63
CC(1/2)	0.998	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	FluB polymerase in a buffer containing 500 mM NaCl, 50 mM Hepes pH 7.5, 5% glycerol and 2mM Tris(2-carboxyethyl)phosphine (TCEP) was mixed with 5' and 3' ends of vRNA promoter and CTD peptide. Reservoir solution contained 0.1 M bicine pH 9.0, 10% MPD.