5M38
Structure of the TagL peptidoglycan binding domain from EAEC T6SS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.8729 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 211.550, 211.550, 211.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 2.600 |
| R-factor | 0.2176 |
| Rwork | 0.217 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3td5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | |
| Number of reflections | 25065 | |
| <I/σ(I)> | 18.5 | 1.5 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 9 | 9.5 |
| CC(1/2) | 0.999 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | mixing protein solution (5 mg/ml) with 0.1 M imidazole pH 6.5, 1.2 M NaAc |
