5M2N

Crystal Structure of Elongator subunit Elp2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.978980
Spacegroup name	P 61 2 2
Unit cell lengths	79.690, 79.690, 532.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.894 - 2.812
R-factor	0.2014
Rwork	0.200
R-free	0.23650
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.800	2.800
Rmerge	0.143	0.819
Number of reflections	45798
<I/σ(I)>	14.4	2.58
Completeness [%]	99.6	95.7
Redundancy	9.3	7.2
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris pH 7.5; 10% PEG 6K; 1750 mM NaCl