5M2E
Apo structure of Pseudomonas aeruginosa Isocitrate Dehydrogenase, ICD.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 294 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9174 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.884, 95.554, 104.015 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 47.777 - 2.700 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bl5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.598 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.000 |
| High resolution limit [Å] | 2.680 |
| Number of reflections | 46653 |
| <I/σ(I)> | 16.2 |
| Completeness [%] | 98.4 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 292.15 | 0.1M Sodium acetate, 30% PEG300 |
