5M25
Modulation of MLL1 Methyltransferase Activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.714, 85.917, 39.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.959 - 2.430 |
R-factor | 0.1795 |
Rwork | 0.176 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eg6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.196 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.960 | 2.560 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.090 | |
Number of reflections | 19553 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 98.8 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD 0.02 M carboxylic acid 0.1 M bicine/Trizma base pH 8.5 |