5M1R
X-ray structure of human G166D PGK-1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.872900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.721, 105.570, 50.002 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 52.780 - 1.640 |
| R-factor | 0.16601 |
| Rwork | 0.164 |
| R-free | 0.20095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.667 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.740 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.097 | 0.570 |
| Number of reflections | 44562 | |
| <I/σ(I)> | 7.84 | 1.82 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.993 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | Sodium Citrate, 1.6 M |
