5M1O

Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Cobalt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	42.937, 53.895, 69.751
Unit cell angles	90.00, 91.62, 90.00

Refinement procedure

Resolution	42.920 - 1.600
R-factor	0.1868
Rwork	0.185
R-free	0.22400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.920	42.920	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.072	0.028	0.725
Rmeas	0.092
Rpim	0.057
Total number of observations	86303
Number of reflections	40243
<I/σ(I)>	6.6
Completeness [%]	95.7	90.1	89.5
Redundancy	2.1	2.2	2
CC(1/2)	0.997	0.998	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium Tartrate, 0.1 M Bis-tris pH 5.5-6.5, 20% PEG 3350