5M1B
Crystal structure of C-terminally tagged apo-UbiD from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 210.187, 210.187, 109.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 3.150 |
| R-factor | 0.22384 |
| Rwork | 0.221 |
| R-free | 0.27655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2idb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.626 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.146 | 0.976 |
| Number of reflections | 51401 | |
| <I/σ(I)> | 14.1 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | Crystals grown in 0.1 mM SPG pH 8, 25 w/v PEG 1500 Crystals were cryo-protected using 10 PEG 400 in mother liquor followed by rapid cryocooling. |
