5M0W
N-terminal domain of mouse Shisa 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-04 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.931 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 59.765, 59.765, 48.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.390 |
R-factor | 0.11644 |
Rwork | 0.115 |
R-free | 0.13668 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Single sulphur |
RMSD bond length | 0.014 |
RMSD bond angle | 1.713 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.880 | 1.410 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.030 | 0.456 |
Number of reflections | 18352 | |
<I/σ(I)> | 30.5 | 3.4 |
Completeness [%] | 99.3 | 88.3 |
Redundancy | 6.8 | 3.2 |
CC(1/2) | 1.000 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2.0 M (NH4)2SO4, 100 mM MES pH 6.5, 4% PEG 400 |