5LYW

CRYSTAL STRUCTURE OF HUMAN METHIONINE AMINOPEPTIDASE-2 IN COMPLEX; WITH AN INHIBITOR 6-((R)-2-o-Tolyloxymethyl-pyrrolidin-1-yl)-9H-purine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-06-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	89.780, 100.230, 100.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.130 - 1.690
R-factor	0.172
Rwork	0.171
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	BUSTER-TNT (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.000		1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.057	0.036	1.025
Number of reflections	59218
<I/σ(I)>	15.7	32.88	2.22
Completeness [%]	98.9	50.1	99.9
Redundancy	6.1
CC(1/2)	0.999	0.997	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	24% methanol, 100 mM citrate